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prism 9 0 0 graphpad software  (Tree Star Inc)
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Journal: Cell reports

Article Title: The FBXW7-SHOC2-Raptor Axis Controls the Cross-Talks between the RAS-ERK and mTORC1 Signaling Pathways

doi: 10.1016/j.celrep.2019.02.052

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Co. Ltd (Shanghai, China) N/A Chemicals, Peptides, and Recombinant Proteins MG132 Sigma Cat#: M7449 cycloheximide Sigma Cat#: 01810 Hoechst 33342 ThermoFisher Scientific Cat#: H1399 Trizol Life Technologies Cat#: 15596018 3xHA peptide Sigma Cat#: 92000-76-5 Critical Commercial Assays ATPlite Luminescence Assay Kit PerkinElmer Cat#: 6016943 Annexin V-FITC apoptosis detection Kit BD Cat #: 556547 Cyto-ID autophagy detection kit Enzo Life Sciences ENZ-51031-0050 Deposited Data SHOC2 mutation Genome Reference Consortium http://cbioportal.org SHOC2 structure This paper; Phyre2 Data http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index Experimental Models: Cell Lines H1299 American Type Culture Collection (ATCC) Cat# NCI-H1299 RRID:CVCL_0060 Huh7 Japanese Collection of Research Bioresources JCRB Cat# JCRB0403, RRID:CVCL_0336 HEK293 American Type Culture Collection (ATCC) Cat# 300192/p777_HEK293, RRID:CVCL_0045 MiaPaCa-2 American Type Culture Collection (ATCC) Cat# ACC-733, RRID:CVCL_0428 H358 American Type Culture Collection (ATCC) Cat# NCI-H358, RRID:CVCL_1559 HCT116 American Type Culture Collection (ATCC) Cat# HCT-116, RRID:CVCL_0291 A549 American Type Culture Collection (ATCC) Cat# A549, RRID:CVCL_0023 Oligonucleotides Table S1 This paper N/A Table S2 This paper N/A Recombinant DNA Plasmid: pEBG-GST-SHOC2 This paper N/A Plasmid: pEBG-GST-SHOC2 (T507A) This paper N/A Plasmid: pEBG-GST-SHOC2 (T242A) This paper N/A Plasmid: pEBG-GST-SHOC2 (1-84) This paper N/A Plasmid: pEBG-GST-SHOC2 (1-346) This paper N/A Plasmid: pEBG-GST-SHOC2 (347-582) This paper N/A Plasmid: pcDNA3-3xHA This paper N/A Plasmid: pcDNA3-3xFlag This paper N/A Plasmid: pcDNA3-His-ubiqutin This paper N/A Plasmid: pcDNA3-Flag-FBXW7 This paper N/A Plasmid: pIRES2-HA-FBXW7 This paper N/A Plasmid: pIRES2-HA-FBXW7ΔF This paper N/A Plasmid: pRK5-HA-Raptor Addgene Plasmid #8513 Plasmid: pRK5-HA-Raptor (1-350) This paper N/A Plasmid: pRK5-HA-Raptor (351-900) This paper N/A Plasmid: pRK5-HA-Raptor (901-1335) This paper N/A Software and Algorithms GraphPad Prism software version 5.01 GraphPad https://www.graphpad.com/support/prism-5-updates/ AlphaEaseFC software AlphaEaseFC https://alphaeasefc-v.updatestar.com/en Other Vectashield Antifade mounting medium Vector Laboratories Cat #: H-1200 DMEM, high glucose ThermoFisher Scientific Cat #: 11965-084 RPMI1640 medium ThermoFisher Scientific Cat #: 11875-085 Open in a separate window KEY RESOURCES TABLE SHOC2 is a FBXW7 substrate for degradation upon phosphorylation by the MAPK signal SHOC2 binds to Raptor to inactivate the mTORC1 signal and induces autophagy Raptor binds to SHOC2 to inactivate the RAS-ERK signal and inhibits growth SHOC2 is altered in lung and pancreatic cancers with correlation of poor survival

Techniques: Recombinant, Luminescence Assay, Mutagenesis, Plasmid Preparation, Software